Fig. 4
From: Engineered CRISPR-Cas systems for the detection and control of antibiotic-resistant infections
Schematic illustration of an engineered CRISPR-Cas9 system that targets antibiotic resistance genes. A The plasmid harbouring the mcr-1 gene was destroyed by the conjugative CRISPR-Cas plasmid. B The engineered CRISPR-Cas9 was delivered to the target bacteria via a host-independent conjugative plasmid and continuously disseminated in microflora, affecting specific drug resistance plasmids containing the target sequence. C The engineered CRISPR-Cas9 system limits the conjugation and transformation of drug resistance plasmids in bacteria by targeting DNA. (Reproduced with permission from [81], and created with BioRender.com)