Fig. 3
Thermodynamic, pH stability and PTX release profile of 4WJ-EGFRapt-miR-375-PTX. a Representative annealing curve of 4WJ-EGFRapt-miR-375-PTX measured by RT-PCR. b Tm values of 4WJ, 4WJ-EGFRapt, 4WJ-miR-375, 4WJ-EGFRapt-miR-375, 4WJ-PTX, 4WJ-EGFRapt-PTX, 4WJ-miR-375-PTX and 4WJ-EGFRapt-miR-375-PTX. c Enzymatic stability of 4WJ-EGFRapt-miR-375-PTX. d Stability of 4WJ-EGFRapt-miR-375-PTX in PBS with pH 4.0, 5.5 and 7.4 analyzed by native PAGE electrophoresis. e PTX release from 4WJ-EGFRapt-miR-375-PTX was validated by native PAGE electrophoresis. Data are representative of at least three independent experiments with three replicates