Fig. 4
Uptake efficiency, tumor penetration and in vitro cytotoxicity analysis. a Uptake efficiency comparation between 4WJ-miR-375-PTX and 4WJ-EGFRapt-miR-375-PTX by KYSE-150 cells. b Quantify the nanoparticles in KYSE-150 cells. c Proliferation of KYSE-150 cells after treatment by 4WJ, 4WJ-EGFRapt, 4WJ-miR-375, 4WJ-EGFRapt-miR-375, PTX, 4WJ-PTX, 4WJ-EGFRapt-PTX, 4WJ-miR-375-PTX and 4WJ-EGFRapt-miR-375-PTX. d Western blotting of Bax, Bcl-2, caspase-3, Cyclin A2, Cyclin B1, Cyclin D1 and E-Cadherin in KYSE-150 cells treated with PBS (#1), 4WJ (#2), 4WJ-miR-375 (#3), 4WJ-PTX (#4), 4WJ-miR-375-PTX (#5), PTX (#6), 4WJ-EGFRapt (#7), 4WJ-EGFRapt-miR-375 (#8), 4WJ-EGFRapt-PTX (#9) and 4WJ-EGFRapt-miR-375-PTX (#10). e Permeability comparation of 4WJ-miR-375-PTX and 4WJ-EGFRapt-miR-375-PTX in 3D tumor spheroids. f Inhibitory effects of PTX and nanodrugs on 3D tumor spheroids. Scale bar: 50 μm. Three independent replicates over at least three independent measurements