Fig. 1

Endothelial permeability is enhanced in coronary microvessels of NAFLD mice. Mice were fed a MCD diet for 4 weeks or HFD for 16 weeks to induce NAFLD. A Representative images of liver H&E staining, oil red O staining and B-scan ultrasonographic imaging, n = 6 per group. Scale bar: 200 μm. B Liver triglyceride (TG) content, n = 6 per group. C The ratio of hepatic-renal echo-intensity, n = 6 per group. D, E Plasma levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT), n = 6 per group. F Representative images and the summarized data of Evans blue concentration in heart tissues, n = 6 per group. G Representative fluorescent confocal images of ZO-1/2 (green) with vWF (red) and the summarized data of the Manders overlap coefficient of ZO-1/2 over vWF. The area of interest (AOI) is selected for higher magnification, n = 6 per group. Scale bar, 20 μm. Data are expressed as the mean ± SEM. Statistics: Student t-test, **P < 0.01 vs. the MCS group; ^&&P < 0.01 vs. the ND group