Fig. 10

Knockdown of TGFBRII blocked the TGF-β/SMAD signaling pathway. a Western blot was performed to determine the expression changes of TGFβRII/SMADs pathway-associated proteins in LX2 cells and TGFBRII KD LX2 cells treated with TGFβ or/and miR-6766-3p mimic. b The expressions of p-SMAD2, p-SMAD3 and SMAD4 in LX2 cells and TGFBRII KD LX2 cells with different treatments were detected by immunofluorescent analysis. Green: p-SMAD2/SMAD4. Red: p-SMAD3. Blue: DAPI. (Original magnification, 20×, Scale bars, 40 μm). c The integrated optical density analysis of p-SMAD2, p-SMAD3 and SMAD4 in b. d The expressions of SMAD2, SMAD3, SMAD4, P38 MAPK and ERK1 were verified by qPCR in LX2 cells, TGFβ-induced LX2 cells and TGFBRII KD LX2 cells under the treatment with TGFβ or/and miR-6766-3p mimic. e The expressions of P38 MAPK and ERK1 in LX2 cells and TGFBRII KD LX2 cells with different treatments were detected by immunofluorescent analysis. Green: P38 MAPK. Red: ERK1. Blue: DAPI. (Original magnification, 20×, Scale bars, 40 μm). f The integrated optical density analysis of P38 MAPK and ERK1 in e