Fig. 3
3D-Exo and 2D-Exo limited the progression of liver fibrosis and promoted liver function recovery in fibrotic mouse model. ICR mice were treated with CCl4 intravenously and 56% alcohol gavage for 4 weeks. During the 4th to 8th week, PBS, 2D-Exo and 3D-Exo were injected intravenously, respectively. a Schematic diagram of treatments in mice. The curves of liver function in fibrotic mouse model showed gradual deterioration of liver function. b The serum liver function indexes of mice treated with exosomes were measured with corresponding test kit. c HE staining of livers with different treatment groups showed that the deformed and necrotic liver cells decreased and the degree of inflammatory infiltration was reduced after exosome treatment, especially in 3D-Exo-treated group. (Original magnification, 20×, Scale bar, 200 μm). d Liver images of normal mice (con), and the mice injected with PBS, 2D-Exo or 3D-Exo. Masson staining showed that liver fibrosis was reduced in 2D-Exo- and 3D-Exo-treated mice compared with PBS-treated mice (Original magnification, 20×, Scale bar, 200 μm). e The oil red staining of liver in normal mice and treated mice was observed, and the content of lipid droplets in 3D-Exo-treated mice was significantly reduced. (Original magnification, 20×, Scale bar, 200 μm) Data represent the mean ± SEM. *P < 0.05, **P < 0.01, and ***P < 0.001