Table 1 Primer sequences used for quantitative real-time PCR analysis of stem cell and chondrogenesis-associated specific genes
Target gene | Sequence |
|---|---|
GAPDH | F: TGTTGCCATCAATGACCCCTT |
R: CTCCACGACGTACTCAGCG | |
ACAN | F: TGAGGAGGGCTGGAACAAGTACC |
R: GGAGGTGGTAATTGCAGGGAACA | |
SOX9 | F: TTCATGAAGATGACCGACGA |
R: CACACCATGAAGGCGTTCAT | |
COL2A1 | F: GGCAATAGCAGGTTCACGTACA |
R: CGATAACAGTCTTGCCCCACTTA | |
COL1A1 | F: TCTGCGACAACGGCAAGGTG |
R: GACGCCGGTGGTTTCTTGGT | |
COL10A1 | F: CCAGGTCTGGATGGTCCTA |
R: GTCCTCCAACTCCAGGATCA | |
CD45 | F: ACGAAGCTCTTAGCGTCAGG |
R: CTCTCGGGTGGAGTCTTCTG | |
CD73 | F: CAGTACCAGGGCACTATCTGG |
R: AGTGGCCCCTTTGCTTTAAT | |
CD90 | F: GACAGCCTGAGAGGGTCTTG |
R: CCCAGTGAAGATGCAGGTTT | |
CD105 | F: CACTAGCCAGGTCTCGAAGG |
R: CTGAGGACCAGAAGCACCTC |