Fig. 2

GFP expression, cellular uptake, and mRNA expression of PAR-Lipo/plasmid in MCF-7 cells. A GFP expression in MCF-7 cells after incubation with PAR-Lipo/pEGFP (5:1, w/w), Lipo 2000/pEGFP (1.5:1, w/w) and PEI/pEGFP (2:1, w/w) complexes under hypoxia or nomoxia. Scale bars, 100 μm. B Cellular uptake of PAR-Lipo/Cy-3 DNA by MCF-7 cells under normoxia or hypoxia. Scale bar, 100 μm. C Relative mRNA expression of EGFP under hypoxia or nomoxia. D Schematic diagram of transfection under different conditions. E GFP expression in MCF-7 cells after treatment as described in D. All error bars are expressed as ± SD, n = 4