Recent advances of fluorescent biosensors based on cyclic signal amplification technology in biomedical detection

Table 1 Cyclic signal amplification strategies for fluorescent biosensors

Method	Characteristic	Advantages	Disadvantages	Real samples	References
RCA	Template, Primer, polymerization, amplification, release target	Good amplification effect, single reaction conditions, full use of reaction materials,	Long reaction times, requiring specific amplification template	Cells, serum, tissue, plasma, fecal, beer, drinking water	[23,24,25]
SDR	Hybridization, strand displacement, release target	No enzyme or cofactor, relatively simple experimental conditions, less external interference conditions, economical and cost-effective	Multiple probes or auxiliary strands need to be designed	Cells, serum, urine, milk, river water	[32, 33]
EAA	Hybridization, digestion, release target	No complex instrument, the signal is effective, the reaction is relatively thorough, and the time is short	Harsh reaction conditions, high temperature requirements, high cost of enzymes	Cells, serum, milk, artificial urine, river water	[35, 36]

ISSN: 1477-3155