Fig. 2

Cellular uptake and in vitro BBB penetration study. A Cellular uptake and analysis of mean fluorescence intensity of nanoparticles by U87-MG cells. Scale bar = 100 μm. B Cellular uptake and analysis of mean fluorescence intensity of nanoparticles by bEnd.3 cells. Scale bar = 100 μm. C BBB model by transwell, bEnd.3 cells were seeded onto the upper chamber coated with human plasma fibronectin, U87-MG cells were seeded onto the lower chamber, nanoparticles were added to the media of upper chamber. D Analysis of fluorescence intensity of nanoparticles in the U87-MG cells seeded on to the lower chamber at different time points. E Fluorescence images of cellular uptake of nanoparticles by the U87-MG cells seeded on to the lower chamber at different time points. Scale bar = 100 μm. *** P < 0.001