Fig. 4
Formation of protein corona (PC) of nanoparticles. A Size distribution and zeta potential of Lipo and PC-Lipo. B Size distribution and zeta potential of Ang-Lipo and PC-Ang-Lipo. C Size distribution and zeta potential of Ang-EM and PC-Ang-EM. D Summary and comparison of mean size, PDI and zeta potential of nanoparticles before and after PC formation. E TEM images of nanoparticles after PC formation. Scale bar = 200 nm. F Rations of protein/lipid for nanoparticles after PC formation. G SDS-page analysis of protein profiles of nanoparticles after PC formation. FBS, fetal bovine serum; PC-L, Liposomes with protein corona; PC-A-L, Ang-Lipo with protein corona; PC-A-EM, Ang-EM with protein corona; A-EM, Ang-EM; MP, membrane proteins of U87-MG cells. H Intensity of bands relative to proteins onto surface of nanoparticles (analyzed by ImageJ, x-axis represents molecular weight while y-axis represents the intensity)