Fig. 6

Antitumor efficacy of DTX@Ang-EM and control nanoparticles. A Relative cell viability of U87-MG cells after incubation of different concentration of DTX formulations. B Cell cycle assay by flow cytometry. C Quantitative analysis of cell cycle showing inhibition effects of different formulations. D 3D tumor spheroid after incubation with different formulations. Scale bar = 50 μm. E Bioluminescence of U87-luc cells showing the GBM growth in mice before and after treatment. F Quantitative analysis of bioluminescence intensity showing GBM growth. G Body weight of GBM-bearing mice before and after treatment. H H&E staining and TUNEL analysis of GBM tissues after treatment. Scale bar = 100 μm. ** P < 0.01, *** P < 0.001, **** P < 0.0001