Fig. 5

Anti-tumor effect of MnP@Lip combined with OXA in 4T1 orthotopic tumor model. A Treatment protocol of MnP@Lip combined with OXA in anti-tumor therapy. B Tumor growth curves of different treatment groups (n = 5). C Tumor weight change after receiving different treatments (n = 5). D Tumor weight change during the trial. E Effect of treatment on liver and kidney functions. F Immunohistochemical and immunofluorescence analysis of Ki67, CD8⁺ and CD4⁺ cells (n = 3). 1, 2, 3 and 4 represent PBS group, MnP@Lip group, OXA group and OXA + MnP@Lip group, respectively. The scale bars are 20 μm. The data of Figure B, Figure C, ASL, ALT and Ki67 satisfying normality, thus One-way ANOVA (including Tukey post-hoc test) or Student's t test was applied. The data of UREA, BUN, CD8⁺ and CD4⁺ satisfying non-normality or heterogeneity variance, thus Kruskal–Wallis test (including Bonferroni post-hoc correction) was applied