Fig. 2
A Uptake of (C16Im)2(C4O)/DOPE/DSPE-PEG/OPA1 lipoplexes labeled with the fluorescent dye DiR’ (red channel) into MEFs wt and MEFs OPA1-KO MEFs (labeled with LysoTracker (green channel) at different incubation times. Yellow colors mean colocalization between channels. Scale bars are 10 μm. B Cell viability of MEFs wt (left) and OPA1-KO MEFs (right) upon incubation with (C16Im)2(C4O)/DOPE/DSPE-PEG/OPA1 lipoplexes at ρeff = 2.5 and 4 and Lipofectamine2000/OPA1 at ρeff = 4 as a positive control. Student’s t-test was performed to measure the significance of statistical difference between the different groups and the negative control (in the absence of treatment, dashed line). (*p < 0.05 was considered statistically significant)