Fig. 2

Lipo-RSV promoted macrophage repolarization from M1- to M2-like phenotype. A Flow cytometry analysis of the M1-like subset (F4/80⁺CD86⁺) and M2-like subset (F4/80⁺CD206⁺). Statistical analysis of the percent of B M1Φ (F4/80⁺CD206⁺) or C M2Φ (F4/80⁺CD86⁺).The mRNA leavel of D–F M2-related markers (CD206, Arg-1 and Chil3), and G–I M1 macrophage markers (CD86, iNOS and CCR7) in the activated macrophages. J Lipo-RSV upregulate CD206 and downregulate iNOS and CD86 protein. K Lipo-RSV promoted the phosphorylation of STAT3 and inhibited the phosphorylation of STAT1. Data are presented as mean ± SD (n = 3); ns, no significance, **P < 0.01, ***P < 0.001, ****P < 0.0001. R: RSV (15 μM); LR: Lipo-RSV (equal to 15 μM RSV)