Fig. 3

Anti-inflammatory effect of Lipo-RSV in vitro. A–E ELISA analysis of the levels of IL-1β, IL-6, TNF-α, IL-12, and IL-10. F–J mRNA levels of IL-1β, IL-6, TNF-α, IL-12, and IL-10 by qRT-PCR. K The down-regulation of IL-1β, IL-6, and TNF-α in M1Φ after treatment. L Expression of the NF-κB pathway-related proteins after treatment. M Expression of the inflammasome-related proteins after treatment. Data are presented as mean ± SD (n = 3); ns, no significance, **P < 0.01, ***P < 0.001, ****P < 0.0001. R: RSV (15 μM); LR: Lipo-RSV ( equal to 15 μM RSV)