Fig. 5

Lipo-RSV ameliorates Periodontitis. A Schematic illustration of P.g LPS-induced periodontitis in Balb/c mice and the treatment regimen. B IL-1β and IL-6 expression in the gingival after treatment. (a) Western blotting assay of IL-1β and IL-6 level in the gingiva, (b) The IL-1βand IL-6 levels of the gingiva were qualified by qRT-PCR assay (n = 3). C p65, p-p65, and TNF-α expression in the gingival after treatment. (a) Western blotting results, (b) The TNF-α levels of the gingiva were qualified by qRT-PCR assay (n = 3). D NLRP3, COX2, and caspase1 expression in the gingival after treatment. E HE staining of the gingiva. Red dashed boxes indicate epithelial junctions, and red arrows indicate infiltrating inflammatory cells. R: dental root; AB: alveolar bone. F Representative figures as indicated by alveolar bone loss and root exposure examined by methylene blue staining. a MH: minocycline hydrochloride, b PD: periodontitis, c N: normal, d R: RSV, e LR: Lipo-RSV. G Statistical analysis of the alveolar bone resorption length. Data are presented as mean ± SD (n = 3); ns, no significance, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001