Fig. 6

Identification of macrophages in periodontitis-associated soft tissue lesions. A (a) Western blotting results of CD206, iNOS, and CD86 expression in the gingival tissues. (b, c) The CD206 and CD86 levels in gingiva were qualified analyzed by WB and ImageJ (n = 3). B (a) Western blotting results of the level of p-STAT1 and p-STAT3. (b, c) The p-STAT1 and p-STAT3 levels in gingiva were qualified analyzed by WB and ImageJ (n = 3). C Macrophages stained for F4/80 (Cy3), CD206 (AF488), iNOS (Cy5), and cell nuclear (DAPI). Scale bar 10 μm (a–e), scale bar 50 μm (f–t). R: RSV, LR: Lipo-RSV. Data are presented as mean ± SD (n = 3); ns, no significance, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001