Fig. 10

A novel nanomedicine based on the self-assembling among Cu²⁺, DOX, and NLG919 for chemotherapy sensitized immunotherapy. a Schematic diagram of the synthetic route and in vivo working mechanism of Cu-DON. b Immunofluorescence images of CRT and HMGB1 in 4T1 cells after different treatments. Scale bar, 25 μm. c Z-stack CLSM images of 4T1 tumor spheroids after being treated by DOX and Cu-DON. Scale bar, 200 μm. d, e Tumor volume change curves of the primary tumor and distant tumor volume