Fig. 2

The infiltration capacity of immune cells in distal tumor sites at varied time points after PTT. a Schematic illustration of PLOV-based PTT to suppress tumor growth at distal tumor. b Immunofluorescence of CD8+ T cells (FITC labeled anti-CD8+) in tumor tissues of different treatment groups. The scale bar is 100 μm. c Flow cytometry analysis of CD4+ and CD8+ T cells in the distal tumor tissues under various treatments. d–f The changes of various cytokines in the distal tumor tissues of mice bearing H22 tumors, including TNF-α (d), IL-6 (e) and IL-12p70 (f) were examined by ELISA at day 1, day 5 and day 10 after respective treatment (PBS, surgical resection and PTT). Data are given as mean ± SD (n = 7). Statistical analysis was conducted by the Student t test for two groups and the one-way ANOVA for multiple groups, and the statistical significance was set as *P < 0.05