Fig. 4

siRNA@PLOV-based PTT combined with anti-PD-1 to suppress lung metastasis after eliminating 4T1 primary tumor. a Schematic of siRNA@PLOV-based PTT plus anti-PD-1 to suppress lung metastasis in fLuc-4T1 primary model. b The tumor volume of mice (N = 7) with inoculation of fLuc-4T1 orthotopic tumor after receiving different treatments. c The survival curve of mice in the fLuc-4T1 orthotopic tumor model with different treatment regimens. d The lungs excised from mice in fLuc-4T1 orthotopic tumor model to evaluate the inhibitory effect on pulmonary metastasis. e The H&E staining of tumor tissues isolated from above mice. The tumor areas were marked by red circles. The scale bar is 400 μm. f Morbidity-free survival of different groups of mice with metastatic fLuc-4T1 tumors in after various treatments indicated to eliminate their primary tumors. Data are given as mean ± SD, N = 7. Statistical analysis was conducted by the one-way ANOVA for multiple groups, and the statistical significance was set as *P < 0.05; **P < 0.01; ****P < 0.001