Fig. 6

A Micrographs showing cellular uptake of DiO/gADNVs(+) and DiO/Lips(+) by confocal laser scanning microscope (CLSM). DAPI (blue) was the nuclear staining channel; DiO (green) was the channel of gADNVs(+) and Lips(+); DiI (red) was the cell membrane staining channel. B The mean fluorescence intensity of DiO/gADNVs(+) and DiO/Lips(+). C The cell damage effect of 30 days stored ICG(+), ICG/Lips(+) and ICG/gADNVs(+) after NIR laser irradiation. D Body weight changes of each group during phototherapy. E Temperature rising situation of each group after NIR irradiation. F Tumor volume changes of each group during therapy. G H&E staining and TUNEL staining of tumor tissue in each group. Scale bar is 50 μm. Data in (B) represent mean values ± SD, n = 3. Data in D–F represent mean values ± SD, n = 5. Statistical differences were analyzed by two-tailed Student’s t-test. *p < 0.05, ****p < 0.0001