Fig. 6

In vivo antitumor activity of PBS, GNP, VGB3, and GNP–VGB3 in murine 4T1 mammary carcinoma tumor model. a Schedule for animal experiments. b Tumor growth inhibition; lines, mean tumor volume for each group of six animals per group;  error bars signify ± SEM. n = 6; **P < 0.01, ***P < 0.001; two-way ANOVA (c) The survival rates of 4T1-bearing mice after treatment were illustrated by Kaplan–Meier curves., and d Body weight measurements taken every 5 days until day 32, presented as mean ± SEM. All groups compared with control and analyzed by prism by two-way ANOVA statistical analysis, mean ± SEM and n = 6 (***P < 0.001, **P < 0.01, ns: not significant). e [¹⁸F]-fluorodeoxyglucose (¹⁸F-FDG) PET imaging of mice treated with GNP, VGB3, GNP–VGB3 or PBS at day 32. Representative PET images are shown with arrows indicating 4T1 mammary carcinoma tumors