Fig. 2

MiR-7-5p suppresses the proliferative activity and promotes the apoptosis of MOLM13 and HL-60 cells. A qRT-PCR analysis verifying the transfection efficiency of the miR-7-5p mimics. B MOLM13 and HL-60 cells were cultivated in the intermediary with/without miR-7-5p mimics for 24, 48, 72 and 96 h. The survival rate of the cells was identified by CCK-8 analysis. *p < 0.05 in contrast to the NC mimic group, **p < 0.01 in contrast to the NC mimic group. C Live/dead staining of MOLM13 and HL-60 cells upon with/without miR-7-5p mimics for 24 h. Scale bar: 50 μm. D Flow cytometry was used to identify the programmed cell death of MOLM13 and HL-60 cells posterior to 24 h. Data are described as the average ± SD (n = 3). (**p < 0.01). (E) Typical pictures of the colony formation of MOLM13 and HL-60 cells treated with/without miR-7-5p mimics for 14 days