Fig. 4

Overexpression of OSBPL11 blocked the miR-7-5p-mediated potency on the apoptosis in AML cells. A MOLM13 and HL-60 cells were subjected to NC mimics transfection (a), miR-7-5p mimics (b), OE-OSBPL11 (c) and miR-7-5p mimics + OE-OSBPL11 (d), respectively, and then co-cultured for 24 h. OSBPL11 protein expression levels were measured by immunoblotting. The quantitation data from immunoblotting analysis were analyzed via ImageJ program. B OSBPL11 expression partly counteract the miR-7-5p-mediated suppressive potency on the proliferative activity of MOLM13 and HL-60 cells. C Live/dead staining of MOLM13 and HL-60 cells under different treating for 24 h. Plotting scale: 50 μm. a NC mimics, b miR-7-5p mimics, c OE-OSBPL11, d miR-7-5p mimics + OE-OSBPL11. D Representative images of colony formation of MOLM13 and HL-60 cells treated with a NC mimics, b miR-7-5p mimics, c OE-OSBPL11, d miR-7-5p mimics + OE-OSBPL11 for 14 days, respectively. E Flow cytometry was employed to identify the programmed cell death of MOLM13 and HL-60 cells after 24 h. Data are expressed as the average ± SD (n = 3). *p < 0.05 in contrast to the NC mimics, **p < 0.01 in contrast to the NC mimics. F a NC mimics, b miR-7-5p mimics, c OE-OSBPL11, d miR-7-5p mimics + OE-OSBPL11 were co-cultured with MOLM13 and HL-60 cells for 24 h, protein expression of PI3K/AKT/mTOR signal path biomarkers were identified via immunoblotting. The quantitation data from immunoblotting analysis were analyzed via ImageJ program, which were expressed as mean ± SD (n = 3)