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Fig. 5 | Journal of Nanobiotechnology

Fig. 5

From: Bone mesenchymal stem cell-derived exosomal microRNA-7-5p inhibits progression of acute myeloid leukemia by targeting OSBPL11

Fig. 5

Isolation and characterization of exosomes from BMSCs. A Representative morphology of BMSCs. B Flow cytometry assay revealed that BMSCs were positive for mesenchyma lineage biomarkers (CD73, CD90 and CD105), negative for hematopoiesis and endothelium biomarkers (CD34, CD11b, CD19, CD45), and negative for HLA-DR. C TEM presented the morphological status of BMSCs-originated exosomes. Scale bar:100 nm. D Nanoparticle tracing assay of the BMSCs-originated exosomes. E Immunoblotting assay of superficial biomarkers CD9, CD63 and Tsg101 expressing standardized to Calnexin in the BMSCs-originated exosomes. F Images of BMSCs-exosomes labeled with PKH-67 (green) taken up by MOLM13 and HL-60 were analyzed via CLSM. Scale bar: 20 μm

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Journal of Nanobiotechnology

ISSN: 1477-3155

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