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Fig. 2 | Journal of Nanobiotechnology

Fig. 2

From: 3D hanging spheroid plate for high-throughput CAR T cell cytotoxicity assay

Fig. 2

Design and fabrication of 3DHSP. A Components of the 3DHSP, a hanging dripper, and a spheroid separation plate. The hanging dripper was 3D-printed, while the separation plate was fabricated by the assembly of poly (methyl methacrylate) (PMMA) and polydimethylsiloxane (PDMS) layers with hole structures. Structures and dimensions of the hanging dripper (B) showing its side view and the spheroid separation plate (C) showing the top, bottom, and side views. D Real image of the 3DHSP. E Schematic describing tumor spheroid formation and CAR T assay in a 3DHSP

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