Fig. 5
From: 3D hanging spheroid plate for high-throughput CAR T cell cytotoxicity assay
Effect of 3DHSP tilt angle on the separation of remaining tumors. A Optical and fluorescent images of untreated spheroids stained with calcein-AM (green) and ethidium homodimer-1 (red) at 3DHSP tilt angles of 0°, 10°, 20°, 30°, and 40°. Scale bar: 300 μm. B Areas of BT474 spheroids at 3DHSP tilt angles of 0°, 10°, 20°, 30°, and 40°. C Fluorescence intensity of live/dead cells in tumor spheroids at different tilt angles. D Optical and fluorescent images of HER2-CAR-T cell-treated spheroids stained with calcein-AM and ethidium homodimer-1 at 3DHSP tilt angles of 0°, 10°, 20°, 30°, and 40°. Scale bar: 200 μm. E Areas of BT474 spheroids at 3DHSP tilt angles of 0°, 10°, 20°, 30°, and 40°. F Fluorescence intensity of live/dead cells in tumor spheroids at 3DHSP tilt angles of 0°, 10°, 20°, 30°, and 40°. Student’s t-test: *p < 0.05, ***p < 0.001; NS: not significant