Table 1 Advantages of the 3DHSP over other devices in screening of effects of immune cytotoxic cells on tumor spheroids
From: 3D hanging spheroid plate for high-throughput CAR T cell cytotoxicity assay
Methods | Agarose-coated well | 3DHSP | |
|---|---|---|---|
Spheroid morphology | Uniform | Uniform | Uniform |
Easiness in separating dead cells from spheroids | Cumbersome (pipetting) | Cumbersome (pipetting) | Easy |
Loss of spheroids | Often | Often | Rare |
Live/dead staining | Required | Required | Not required |
Screening of effect of anti-cancer drugs or immune cytotoxic cells on tumor spheroids | Difficult | Difficult | Easy |