Fig. 2

A The survival rate of 4T1 cells after the treatment with different concentrations of DOX, ICG and DIMP for 24 h. B Live/dead assay of cells and C quantification results of living cells. D Confocal fluorescence imaging showed the uptake of DIMP in 4T1 cells (Scale bar: 10 μm). The distribution curves (E, F) and mean fluorescence intensity (MFI) of DOX and ICG at 2 h and 6 h, respectively (G). H Flow cytometry analysis of the cellular uptake of DIMP for 0.5 h, 1 h, 2 h, 4 h and 6 h. I ROS generation in 4T1 cells incubated with ICG, DOX and DIMP with or without laser irradiation and J the results of average fluorescence intensity. (Scale bar: 20 μm). The data showed mean ± SD (n = 3). *: P < 0.05, **: P < 0.01 and ***: P < 0.001