Fig. 3
In vitro CDT effect and ICD induction. a Cell viability of saline, H2O2 (100 μM), NA0, NAc or dNAc (Fe concentration = 50 μg/mL) treated 4T1 cancer cells in the absence or presence of H2O2 (100 μM), followed by treatment of NIR-II laser (1 W/cm2) for 6 min. b Fluorescence images of 4T1 cancer cells treated with NA0, NAc or dNAc (50 μg/mL) in the presence of H2O2 (100 μM), followed by treatment of NIR-II laser (1 W/cm2) for 6 min (the generated ·OH showed green fluorescence signals). c Extracellular ATP levels for 4T1 cancer cells after different treatments. d WB assay of the expression level of CRT for 4T1 cancer cells after different treatments. e Detection of HMGB1 extracellular release from 4T1 cancer cells after different treatments