Fig. 4

In vivo ICD and DC maturation. a Fe content assay in tumors of mice after intravenous injection of saline, NA₀, NAc and dNAc at different timepoints. b Thermal imaging of mice after injection of saline, NA₀, NAc and dNAc with treatment of NIR-II laser (1 W/cm²) for different time. c Temperature of tumors for NA₀, NAc and dNAc-injected mice at different time-points with treatment of NIR-II laser (1 W/cm²). Immunofluorescence staining images of CRT (d) and HMGB1 (e) of tumors. CRT and HMGB1 stained with corresponding antibody showed green fluorescence signals. f Relative ATP levels in tumors of mice after intravenous injection of NA₀, NAc and dNAc with or without treatment of NIR-II laser (1 W/cm²) for 10 min. g Flow cytometry assay of matured DCs in tumor-draining lymph nodes. h Populations of matured DCs (CD80⁺CD86⁺) in tumor-draining lymph nodes