Fig. 4
Antibody isotyping assay and cytokine detection. A Antibody isotyping assay of IgG1, IgG2a, IgG2b, IgG3, IgA and IgM isotypes elicited by ferritin; B YaH3F; C YaH4F; D YaH5F; E combination; F PBS; G YaHF. Detection of IFN-γ (H), IL-2 (I) and IL-4 (J) levels in antisera elicited by ferritin, YaH3F, YaH4F, YaH5F, combination immunity, PBS and YaHF. Detection of IFN-γ (K), IL-2 (L) and IL-4 (M) levels in cultured supernatant of spleen lymphocytes were cultured for 24 h with stimulator. Comb stands for combined immunization. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001