Fig. 5
Detection of the titre of neutralizing antibody induced by self-assembled immunogen and the immune effect on lymphocytes. A The neutralization titre of the antibody was detected by the microneutralization method. Under the condition of the 100TCID50 CDV-11 strain, the half inhibitory dose of neutralizing antibody (ID50) was calculated. B Ferritin, YaH3F, YaH4F, YaH5F, combined immunization, PBS and YaHF induced lymphocyte proliferation stimulated by peptides, haemagglutinin, ConA and LPS. C Representative flow cytometric plots for measuring recruited and/or activated CD3/CD4 and CD3/CD8 in mice vaccinated with ferritin, YaH3F, YaH4F, YaH5F, combined immunization, PBS and YaHF. D Percentage of recruited and/or activated CD3 cells to lymphocytes. Differentiation degrees of CD4 from CD3 E and CD8 from CD3 F are shown. G The killing efficiency of antibody-dependent cell-mediated cytotoxicity (ADCC) compared with Triton X-100. Comb stands for combined immunization. *P < 0.05, **P < 0.01, ***P < 0.001