Fig. 2
From: CRISPR/Cas9 delivery by NIR-responsive biomimetic nanoparticles for targeted HBV therapy
Characterization of NIR-controlled UCNPs-Cas9@CM. TEM (A) and SEM (B) images of UCNPs-Cas9@CM. C Hydrodynamic size distribution of CMs, UCNPs and UCNPs-Cas9@CM. D Zeta potentials of Cas9/sgRNA, UCNPs-PAA, UCNPs-Avidin, UCNPs-Avidin/PCB, UCNPs-Cas9 and UCNPs-Cas9@CM. E SDS-PAGE protein analysis of total cell proteins, UCNPs-Cas9@CM, avidin and Cas9 protein (36 pmol) (from left to right). Samples were stained with Coomassie Blue. F WB analysis of (I) cell lysate and (II) CMs. G Hydrodynamic diameter variations over time of UCNPs-Cas9@CM. H UV–vis absorption (280 nm) of the supernatant of UCNPs-Cas9 was measured using a 808 nm laser at different irradiation times. I CLSM images of cells were incubated with UCNPs-Cas9@CM at 6 h with NIR-/ + irradiation (green: GFP-labeled Cas9; blue: DAPI). Scale bars: 25 μm