Fig. 1
Development and characterization of BCG-Nanocage, and BCG-Nanocage preferentially target macrophages (MΦ), but not T cells, B cells or endothelium. A Schematic diagram of preparation for BCG-Nanocage from BCG. B SDS-PAGE analysis of the protein contents in BCG lysis and BCG-Nanocage lysis from three different batches of samples, proteins were stained by Coomassie Blue. C Size distribution of BCG-Nanocage. D Size distribution changes of BCG-Nanocage during 7 days storage at 4 ℃. E Scanning electron microscopy (SEM) imaging of BCG bacteria. F SEM imaging of BCG-Nanocage. G Transmission electron microscopy (TEM) imaging of BCG-Nanocage. H–I Enlarged TEM imaging of BCG-Nanocage from the indicated area in (G). J Confocal imaging for cellular uptake of GFP-BCG-Nanocage by macrophages from the freshly isolated bronchoalveolar lavage (BAL) fluid of rhesus macaques after 1 h treatment of GFP-BCG-Nanocage, followed with staining using anti-human CD11b-APC antibody and anti-human CD3-PE antibodies, scale bar: 20 μm. K Flow cytometry analysis for cellular uptake of GFP-BCG-Nanocage by macrophages, T cells, B cells and endothelium from the freshly isolated intraepithelium (IEL) of rhesus macaques after 1 h treatment. L Cellular uptake of GFP-BCG-Nanocage by macrophages, T cells, B cells and endothelium from the freshly isolated intraepithelial lymphocytes of rhesus macaques after 1 h treatment, n = 4, ***p < 0.001