Fig. 5

BCG-Nanocage boost immunization in non-human primates enhance IFN-γ and Granzyme B production in Vγ2Vδ2 T cells. Typical flow images for intracellular IFN-γ level in Vγ2+ T cells from PBMC of rhesus macaques before and after BCG immunization or BCG-Nanocage immunization with (A) anti-CD28/anti-CD49d stimulation or (B) co-stimulation with anti-CD28/anti-CD49d and BCG-Nanocage, cells were gated in CD3 + Vγ2 + cells. C Statistical results for co-stimulation using anti-CD28/anti-CD49d and BCG-Nanocage induced changes of intracellular IFN-γ levels in Vγ2+ T cells from PBMC of rhesus macaques before and after BCG or BCG-Nanocage immunization, data were expressed as percentages of control, n = 4, *p < 0.05. Typical flow images for intracellular level of Granzyme B in Vγ2+ T cells from PBMC of rhesus macaques before and after BCG or BCG-Nanocage immunization with (D) anti-CD28/anti-CD49d stimulation or (E) co-stimulation anti-CD28/ anti-CD49d and BCG-Nanocage, cells were gated in CD3+ Vγ2+ cells. F Statistical results for co-stimulation using anti-CD28/anti-CD49d and BCG-Nanocage induced changes of intracellular Granzyme B levels in Vγ2+ T cells from PBMC of rhesus macaques before and after BCG or BCG-Nanocage immunization, data were expressed as percentages of control, n = 4, *p < 0.05