Fig. 4
From: Betulinic acid self-assembled nanoparticles for effective treatment of glioblastoma
BA NPs suppress NFκB-p65 and SP1 signaling through CB1, CB2/Akt pathways. A Western blot assay of main protein expression of signaling pathways after BA NPs treatment in different concentration (0, 5, 10, 15 µg/mL). B, C Representative fluorescent images of p-p65 in U87 cells treated with BA NPs by confocal microscope. D, E Representative fluorescent images of SP1 in U87 cells treated with BA NPs treatment by confocal microscope. F Western blot analysis of the expression of the indicated proteins in U87 cells after treatment with BA NPs, CB1 INH (SR141716A) or CB2 INH (SR144528). G, H. Flow cytometry analysis (G) and quantification (H) of cell apoptosis after treatment with SR141716A or SR144528 prior to BA NPs treatment in U87 cells. ***P < 0.001; **P < 0.01; *P < 0.05