Fig. 5
From: Betulinic acid self-assembled nanoparticles for effective treatment of glioblastoma
Cellular uptake and biodistribution of BA NPs in vitro and in vivo. A SEM pictures of U87 cells incubating with BA NPs for 4 h. Scale bar: 1000 nm. B, C Confocal microscopic pictures of U87 cells incubating with BA-coumarin-6-NPs (BA-C6 NPs) in different times (5, 20, 60 min). ***P < 0.001; **P < 0.01. D Flow cytometry analysis of U87 cells incubating with BA-coumarin-6-NPs in different times (0, 5, 20, 60 min). E The plasma concentration (C6 fluorescence intensity) changed with time after BA NPs injected from caudal vein. F IVIS image of the brain, heart, lung, liver, spleen, and kidney 8 h after IR780 labeled PLGA NPs, BA NPs and free dye injected into mice via tail vein. G, H Confocal microscope images of the tissues harvested from BA-C6 NPs injected mice euthanized at the indicated time points