Fig. 2

Exo-Gel promoted proliferation and migration of mBMSCs in vitro. A In vitro setup to determine if Exo released from Exo-Gel maintain their ability in proliferation and migration of mBMSCs. B The cell viability of mBMSCs cocultured with conditioned medium examined by CCK-8 analysis. Data are expressed as means ± SEM (n = 6). *p < 0.05, Exo-Gel group compared to Gel group. C Proliferation of mBMSCs was confirmed by EDU kit assay (bar = 100 μm). D Quantification of rate of EDU positive cells in C was performed. Data are expressed as means ± SEM (n = 8). (E) Transwell assay to show a representative image of migrating mBMSCs (bar = 200 μm). F Quantitative analysis of migrating cells in E. Data are expressed as means ± SEM (n = 6). G A representative image showing the migration ability of mBMSCs by the scratch test (bar = 500 μm). (H) Quantitative analysis of mBMSC migration rate (G). Data are expressed as means ± SEM (n = 6). *p < 0.05