Fig. 2
Optical absorption, photothermal properties, and free radical scavenging properties of nanocomposites. a UV–vis spectra. b The linear relationship between the optical absorbance at 690 nm of RMnMels and mass concentrations. c Photothermal heating curves of 200 µg/mL RMnMels during 690 nm laser irradiation with various laser densities. d Photothermal heating curves of RMnMels with various concentrations during 690 nm laser irradiation at 500 mW/cm2. e Calcein-AM/PI co-stained images of PC3 cells after different treatments. Scale bar, 20 μm. f PC3 cells viability after different treatments. DPPH (g), O2.− (h), and ·OH (i) scavenging ability of nanocomposites. j ROS fluorescence intensity in RAW 264.7 cells after different treatments. k Effects of RMnMels on oxygen consumption rate of PC3 cells. l Quantification of basal respiration and spare respiratory capacity. n Fluorescence images for detection of intracellular ROS in PC3 tumor tissue slices. Scale bar, 50 μm. All experiments were run at least in triplicate. The statistical differences were quantified using Student’s t test (*p < 0.05, **p < 0.001, ***p < 0.0001)