Fig. 3

RMnMels for M2-to-M1 macrophages repolarization. a RT-PCR analysis of mRNA expression levels in RAW 264.7 cells. b Western blot analysis of protein expression levels in RAW 264.7 cells. ELISA assay for the levels of cytokines in RAW 264.7 cells (c) and PC3 tumor tissues (d) after different treatments. e Representative immunofluorescence images showing the phenotypes of macrophages after different treatments. Scale bars, 20 μm. f Representative immunofluorescence images of CD163 and iNOS in PC3 tumor tissue slices after different treatments. Scale bars, 20 μm. All experiments were run at least in triplicate. The statistical differences were quantified using Student’s t test (*p < 0.05, **p < 0.001, ***p < 0.0001)