Fig. 5
In vivo imaging using SPN-PT. A NIR-II fluorescence images of 143B xenograft mice at different time points post injection of SPN-PT (upper, 100 µg mL−1, 100 µL, i.v.) or SPN-SP (lower, 100 µg mL−1, 100 µL, i.v.). The fluorescence images were acquired under the excitation of 808 nm with a 980 nm filter. Scale bar = 1 cm. B Monitoring of NIR-II fluorescence intensities collected within the tumor region (red circle in A) of 143B tumor bearing mice. Data were shown as mean ± SD, n = 3. (p < 0.05, *; p < 0.01, **; p < 0.001, ***; p < 0.0001, ****). C Two-dimensional (upper) and three-dimensional (lower) PA imaging of 143B tumors in living mice injected with SPN-PT (100 µg mL−1, 100 µL, i.v.) at 1 h, 4 h, 8 h, 24 h. The PA images were acquired at 695 nm. D Average PA intensities within the whole tumor area (white circle in C). (i.v. intravenous injection). Data were shown as mean ± SD, n = 3