Fig. 3

IM@ZP uptake and transfection efficiency in vitro. A Quantitative analysis of fluorescence signal intensity of siRNA-FAM in 4T1 cells incubated with IM@ZP for 1, 2, 4 and 6 h analyzed by flow cytometer. B Quantitative analysis of fluorescence signal intensity of IR792 in 4T1 cells incubated with IM@ZP for 1, 2, 4 and 6 h analyzed by flow cytometer. C CLSM observation of the co-localization of siRNA-FAM (green) and lysosome (red) in 4T1 cells after co-incubation of IM@ZP with 4T1 cells. D CLSM images of 4T1-GFP cells incubated with IM@Z and GFP-siRNA at a 80:1 mass ratio (concentration of siRNA was 80 nM), with NC-siRNA serving as the control. E Flow cytometric analysis of GFP fluorescence curve in 4T1-GFP cells incubated with IM@Z and GFP-siRNA at a mass ratio of 80:1. F Flow cytometric analysis of GFP expression rate in 4T1-GFP cells incubated with IM@Z and GFP-siRNA at different mass ratios. G Western blot analysis of PD-L1 protein in 4T1 cells incubated with IM@Z binding to 40, 80 or 160 nM of PD-L1 siRNA, with NC-siRNA serving as the control. *p  < 0.05