Table 2 In vitro binding affinities of Remicade, C1, C3, and C5 mAb candidates
From: A universal in silico V(D)J recombination strategy for developing humanized monoclonal antibodies
Candidates (mAbs) | wRMSD(Å) | BLItz affinity measurement system | ||
|---|---|---|---|---|
Ka (1/Ms) | Kd(1/S) | KD (M) | ||
C1 | 1.207 | 1.43 × 105 | 1.34 × 10–4 | 9.35 × 10–10 |
C3 | 1.327 | 2.72 × 104 | 1.50 × 10–4 | 5.50 × 10–9 |
C5 | 1.469 | 2.68 × 104 | 4.75 × 10–4 | 1.77 × 10–8 |
rRemicade | 1.137 | 1.86 × 105 | 9.53 × 10–5 | 5.13 × 10–10 |