Fig. 4
From: Functional nanovesicles displaying anti-PD-L1 antibodies for programmed photoimmunotherapy
Biological distribution and PTT of nanomissiles (aPD-L1 NVs-ICG) on tumor in vivo. a FL images of B16-F10 tumor-burdened mice at different points in time after injection of different drugs through tail vein. b The curve of fluorescence signal intensity in tumor area with time. cFluorescence signal intensity of major organs and tumors of the mice in a at 48 h. d Schematic diagram of photothermal treatment for double tumor-bearing mice. e NIR image of the tumor after different treatments under 808 nm laser irradiation (0.75 W/cm2, 8 min). f Tumor volume growth curve for irradiated primary tumors (in left flank) in mice after PTT with different drugs. g The content of mature DC cells in the lymph nodes of mice was detected via analysis of dendritic cell maturation markers (CD11c + CD86 +) by flow cytometry after PTT for 3d. h The content of CD8 + T cells in bilateral tumors of mice was detected via analysis of CD8 + T cells markers (CD3a + CD8 +) by flow cytometry after PTT for 3d. T1 represents the primary tumor at the left posterior back and T2 represents the distal tumor at the right posterior back. i Production of IFN-γ in serum from the mouse determined by ELISA after treated with PTT for 3d. n = 3 ~ 5. P values were calculated by Student's t test (***P < 0.001, **P < 0.01 or *P < 0.05)