Fig. 6

Biological distribution of AAI and photoimmunotherapy on tumor combine nanomissiles with and nanovaccines in vivo. a Fluorescent (FL) images of C57/BL-6 mice at 96 h after the drug was injected into the sole of the left foot. b, c FL images and fluorescence signal intensity of inguinal lymph nodes of the mice in a. d Schematic diagram of photothermal-immunotherapy for double tumor-bearing mice. e Tumor volume growth curve of distant secondary tumors after photoimmunotherapy with different drugs. f Survival curves of the mice after photoimmunotherapy with different drugs. g The content of mature DC cells in the lymph nodes of mice was detected via analysis of dendritic cell maturation markers (CD11c + CD86 +) by flow cytometry after last dose for 3 d. h The content of CD8 + T cells in distant secondary tumors of mice was detected via analysis of CD8 + T cells markers (CD3a + CD8 +) by flow cytometry after last dose for 3 d. i-l The amount of TNF-α, IL-6, IL-12, IFN-γ in serum of the mouse determined by ELISA after last dose for 3 d. n = 3 ~ 5. P values were calculated by Student's t test (***P < 0.001, **P < 0.01 or *P < 0.05)