Fig. 1

BMSCs Secrete Exosomal miRNAs. A Electron microscopic analysis of Exos secreted by BMSCs. Scale bar: 200 nm. B Particle size distribution of vesicles secreted from BMSCs, as measured by NanoSight analysis. C The concentrations of the exosome (Exo)-specific marker TSG101 and extracellular vesicle-related protein markers HSP70, CD63, and CD9 were measured by Western blot analysis. The blots are representative of 3 replicates of independent experiments, each with 2 samples. D BMSCs transfected with a Cy3-labeled miR-223 mimic were co-cultured with BMSCs in a transwell (membrane pore size: 0.4 μm) plate. E Exos from BMSCs were labeled with PKH26 and then added to BMSC cultures. The results demonstrate the effects of the EV secretion inhibitor GW4689 (10 mM) on exosome-dependent miRNA delivery from BMSCs into recipient BMSCs. Data represent means ± SD. ***p < 0.001