Fig. 4

Exosomes modulate BMSC differentiation through exosomal miRNAs. A Exos were collected from normal BMSCs and DM BMSCs after siRNA-mediated knockdown of Drosha and then cultured with untreated N-BMSCs or DM-BMSCs. After 48 h, the N-BMSCs and DM-BMSCs were collected and the protein expression levels of osteogenic molecules were measured by Western blotting. B Western blot analysis of Col-1, Runx-2, ALP, and Sp7 expression in N-BMSCs and DM-BMSCs cultured with Exos both with or without Drosha knockdown. n = 3 in each group. C–F Quantitative analysis of Col-1, Runx-2, ALP, and Sp7 expression by Western blotting. n = 3 in each group. *p < 0.05; **p < 0.01; ***p < 0.001. Data represent means ± SD