Fig. 10

PLGA nanoparticles protect cultured neurons. Histogram depicting dose-dependent decrease in the viability of mouse cortical cultured neurons following 24 h exposure with oligomeric human Aβ_1–42 compared to control (CTL) neurons as revealed by MTT (A) and LDH (B) assays. Histograms showing protection of mouse cultured neurons following co-treatment of 10 µM Aβ_1–42 with 25 µM PLGA over 24 h as detected with MTT (C) and LDH (D) assays. Histograms showing protection of primary cortical neurons following treatment with Aβ_1–42 samples collected after 25 µM PLGA-mediated spontaneous attenuation of Aβ_1–42 aggregation (E, F) and disassembled of matured Aβ_1–42 fibers (G, H) at 37 °C as detected with MTT (E, G) and LDH (F, H) assays. Immunoblots and histograms showing that protective effects following attenuation of spontaneous Aβ aggregation by PLGA are associated with a decrease in the levels of Phospho-Tyr²¹⁶ GSK-3β (I), Phospho-ERK1/2 (J) and Phospho-tau (K) induced by 10 µM Aβ_1–42 alone. All results, which are presented as means  ±  SEM, were obtained from three to five separate experiments. **p  < 0.01, ***p  < 0.001